in the Registry Editor, Go to HKEY_LOCAL_MACHINE >SYSTEM>CurrentControlSet >services>USBSTOR
Double click on the start key which is in the right side. Then it will open a window, you will see Value Name and Value data. In the value data field , you can change the value to 4 to disable USB ports. When you change that value to 3 it will enable USB Ports.
Windows Registry Editor Version 5.00
[HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Services\UsbStor]
"start"=dword:00000004
Change the Save as type to "All Files" and give a name with .reg (ex: Disable_usb.reg) as shown in below figure.
Now,you can also make reg file for enabling USB Port by changing dword value to 00000003.
By double clicking these two reg files, it can be easily Disable and Enable USB Ports. Remove the USB Drive and again plug for applying these settings. I have made these Registry Files for you so, if you unable to make these files download it from here link.
There is a small application available for Disabling and Enabling USB Ports called USB Port Locked 2.0. Using this application, it may be easily done.
Get USB Port Locked 2.0 from here link
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